Thermo Scientific Orbitrap Fusion / UltiMate 3000 nanoLC System
Equipment overview
The Thermo Scientific Orbitrap Fusion Liquid Chromatography/Tandem Mass Spectrometer is a high-resolution, accurate-mass (HRAM) multi-attribute tribrid mass spectrometer designed to deliver exceptional performance, operational versatility, and robust quantification and qualitative capabilities for complex proteomics, small molecule analysis, and structural elucidation.
Major applications
Proteomics (global, quantitative, and PTM profiling), metabolomics, lipidomics, macromolecular complex analysis, and small molecule structure elucidation.
Specifications
- Liquid Chromatography
- Thermo Scientific UltiMate 3000 RSLCnano System
- • Mass Spectrometer
- Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer
- • Ionization Modes
- Electrospray Ionization (ESI) and Nano-Electrospray Ionization (NSI)
- • Configuration
- Quadrupole-Linear Ion Trap-Orbitrap
- • Acquisition Modes
- Full MS Scan, Product Ion Scan (MS2/MSn), Data-Dependent Acquisition (DDA), Data-Independent Acquisition (DIA), targeted SIM (tSIM), targeted MS2 (tMS2), and Multiple Dissociation Techniques (CID, HCD, ETD)
- • Mass Range
- m/z 50–2,000 (standard); m/z 200–4,000 (high mass); up to m/z 6,000 without precursor ion selection
- • Mass Resolution
- Up to 450,000 (FWHM) at m/z 200
- • Maximum Scan Speed
- Up to 20 Hz (Orbitrap)
- • Mass Accuracy
- <3 ppm RMS using external calibration; <1 ppm RMS using internal calibration.
- • Software
- Orbitrap Fusion Instrument Control Software, Chromeleon CDS, Xcalibur